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Contents

9. Contrast methods for

Leica DM4000 B/DM4500 B/

DM4500 P/DM5000 B ................................ 60

9.1 Transmitted light ........................................ 60

9.1.1 Bright field ......................................... 60

9.1.2 Phase contrast.................................. 60

9.1.3 Dark field............................................ 61

9.1.4 Polarization........................................ 61

9.1.4.1 Manual method ............................. 61

9.1.4.2 DM4500 P - examinations

in polarized transmitted light...... 62

9.1.4.3 Motorized method ........................ 68

9.1.4.4 Combined methods....................... 68

9.1.5 Differential interference

contrast ............................................ 68

9.1.5.1 DM4500 B/DM4500 P .................... 68

9.1.5.2 DM5000 B........................................ 69

9.2 Fluorescence.............................................. 70

10. Contrast methods for

Leica DM4000 M ........................................ 71

10.1 Incident light .............................................. 71

10.1.1 Bright field ....................................... 71

10.1.2 Dark field.......................................... 71

10.1.3 Polarization...................................... 72

10.1.4 Interference contrast .................... 73

10.2 Transmitted light ........................................ 73

10.2.1 Bright field ....................................... 73

10.2.2 Polarization...................................... 73

11. Troubleshooting......................................... 74

12. Care of the microscope ........................... 77

12.1 Dust cover................................................... 77

12.2 Cleaning....................................................... 77

12.3 Handling acids and bases ....................... 78

13. Essential wear and spare parts ............. 79

14. Abbreviations and pictograms ............... 80

15. Index ............................................................ 81

16. EU Declaration of Conformity................. 82

1 2 3 4 5 6 7 8 9 10 11 ... 163 164

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Sharp DM-4500 Istruzioni per gli Interventi Pagina 6